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1.2% FA Gel Preparation
NB: the gel must be cast in the fume hood
To prepare FA gel (1.2% agarose), mix the following in a specified conical flask:
·1.2 g agarose
·10 ml 10x FA gel buffer (see composition below)
·Add RNase-free water to 100 ml
Heat the mixture to melt agarose. Cool to a holdable temperature. Add 1.44 ml of 37% formaldehyde and either 3 µl of SYBRgreen II stock solution or if preferred, Ethidium Bromid...