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Created on: 08-Mar-2015. Updated on: 08-Mar-2015.

Category: Nucleic Acids.

Colony qPCR (screening of successful ligations)2

Posted by: dami82, UIC, Chicago, IL, http://www.biotechworld.it/portfolio/fantini



This procedure allows the screening of a high number of single transformant colonies (E.coli) for the presence (and/or the length) of a specific DNA sequence. In order to get reliable results, it is advisable to set up qPCR reactions starting from O/N cultures rather than from E.coli colonies. Briefly, selected colonies from a plate should be inoculated in 1-5 mL of LB and grown O/N. O/N cultures will be assayed and t...

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